Mediator protein mutations that selectively abolish activated transcription.

  title={Mediator protein mutations that selectively abolish activated transcription.},
  author={Lawrence C. Myers and Claes M. Gustafsson and Kathleen C. Hayashibara and Patrick O. Brown and Roger D. Kornberg},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={96 1},
Deletion of any one of three subunits of the yeast Mediator of transcriptional regulation, Med2, Pgd1 (Hrs1), and Sin4, abolished activation by Gal4-VP16 in vitro. By contrast, other Mediator functions, stimulation of basal transcription and of TFIIH kinase activity, were unaffected. A different but overlapping Mediator subunit dependence was found for activation by Gcn4. The genetic requirements for activation in vivo were closely coincident with those in vitro. A whole genome expression… CONTINUE READING


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