Mediated amperometric determination of xylose and glucose with an immobilized aldose dehydrogenase electrode.

@article{Smolander1992MediatedAD,
  title={Mediated amperometric determination of xylose and glucose with an immobilized aldose dehydrogenase electrode.},
  author={M Smolander and H L Livio and Leena R{\"a}s{\"a}nen},
  journal={Biosensors & bioelectronics},
  year={1992},
  volume={7 9},
  pages={637-43}
}
An enzyme electrode was constructed for amperometric determination of xylose and glucose. The electrode is based on the PQQ-dependent membrane-bound aldose dehydrogenase (ALDH) from Gluconobacter oxydans. ALDH was covalently immobilized on a graphite electrode. Immobilized dimethylferrocene, soluble ferrocene carboxylic acid and phenazine methosulphate were used as electron transfer mediators. When xylose was measured electrochemically using an electrode modified with ALDH and dimethylferrocene… CONTINUE READING