Media selection for refolding of thermolysin by use of immobilized preparation.

@article{Nohara2000MediaSF,
  title={Media selection for refolding of thermolysin by use of immobilized preparation.},
  author={Daisuke Nohara and Yasuhiro Senga and Masaaki Matsubara and Tokiko Sakai},
  journal={Journal of bioscience and bioengineering},
  year={2000},
  volume={89 2},
  pages={188-92}
}
Selection of the most effective medium for the correct refolding of thermolysin was performed. Thermolysin that had been denatured with 6 M guanidinium chloride at pH 2.0 could not be recovered to its activity larger than ca. 10% even when the denaturant was diluted with a conventional buffer solution. The amount of activity recovered by this method decreased with time. The recovered activity was ca. 20% at most where 1 M calcium chloride or 1.6 M calcium acetate was employed as the refolding… CONTINUE READING