Mechanistic studies on the mononuclear ZnII-containing metallo-beta-lactamase ImiS from Aeromonas sobria.

@article{Sharma2006MechanisticSO,
  title={Mechanistic studies on the mononuclear ZnII-containing metallo-beta-lactamase ImiS from Aeromonas sobria.},
  author={Narayan P. Sharma and Christine E Hajdin and Sowmya Chandrasekar and Brian Bennett and K Yang and Michael W Crowder},
  journal={Biochemistry},
  year={2006},
  volume={45 35},
  pages={10729-38}
}
In an effort to understand the reaction mechanism of a B2 metallo-beta-lactamase, steady-state and pre-steady-state kinetic and rapid freeze quench electron paramagnetic resonance (EPR) studies were conducted on ImiS and its reaction with imipenem and meropenem. pH dependence studies revealed no inflection points in the pH range of 5.0-8.5, while proton inventories demonstrated at least 1 rate-limiting proton transfer. Site-directed mutagenesis studies revealed that Lys224 plays a catalytic… CONTINUE READING

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