Mechanistic studies on aggregation of polyethylenimine-DNA complexes and its prevention.

@article{Sharma2005MechanisticSO,
  title={Mechanistic studies on aggregation of polyethylenimine-DNA complexes and its prevention.},
  author={Vikas Kumar Sharma and Mini Thomas and Alexander M. Klibanov},
  journal={Biotechnology and bioengineering},
  year={2005},
  volume={90 5},
  pages={
          614-20
        }
}
Aggregation of polyethylenimine (PEI)-DNA complexes severely undermines their utility for gene delivery into mammalian cells. Herein we undertook to elucidate the mechanism of this deleterious phenomenon and to develop rational strategies for its prevention. The effect of temperature, surfactants, complex concentration, ionic strength, viscosity, and pH on the time course of this aggregation was systematically examined. The aggregation process was completely inhibited by 2.5% polyoxyethylene… CONTINUE READING
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