Mechanistic studies of Rhodobacter sphaeroides Me2SO reductase.

@article{Cobb2005MechanisticSO,
  title={Mechanistic studies of Rhodobacter sphaeroides Me2SO reductase.},
  author={Nathan Cobb and Thomas Conrads and R. F. Hille},
  journal={The Journal of biological chemistry},
  year={2005},
  volume={280 12},
  pages={11007-17}
}
Studies of the molybdenum-containing dimethyl sulfoxide reductase from Rhodobacter sphaeroides have yielded new insight into its catalytic mechanism. A series of reductive titrations, performed over the pH range 6-10, reveal that the absorption spectrum of reduced enzyme is highly sensitive to pH. The reaction of reduced enzyme with dimethyl sulfoxide is found to be clearly biphasic throughout the pH range 6-8 with a fast, initial substrate-binding phase and substrate-concentration independent… CONTINUE READING