Mechanistic insights on riboflavin synthase inspired by selective binding of the 6,7-dimethyl-8-ribityllumazine exomethylene anion.

@article{Kim2010MechanisticIO,
  title={Mechanistic insights on riboflavin synthase inspired by selective binding of the 6,7-dimethyl-8-ribityllumazine exomethylene anion.},
  author={Ryu-Ryun Kim and Boris Illarionov and Monika Joshi and Mark Cushman and Chan Yong Lee and Wolfgang Eisenreich and Markus Fischer and Adelbert Bacher},
  journal={Journal of the American Chemical Society},
  year={2010},
  volume={132 9},
  pages={2983-90}
}
Riboflavin synthase catalyzes the transfer of a four-carbon fragment between two molecules of the substrate, 6,7-dimethyl-8-ribityllumazine, resulting in the formation of riboflavin and 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione. Earlier, a pentacyclic adduct formed from two substrate molecules was shown to be a catalytically competent intermediate, but the mechanism of its formation is still poorly understood. The present study shows that the recombinant N-terminal domain of riboflavin… CONTINUE READING