Mechanistic insight into pentatricopeptide repeat proteins as sequence-specific RNA-binding proteins for organellar RNAs in plants.

@article{Nakamura2012MechanisticII,
  title={Mechanistic insight into pentatricopeptide repeat proteins as sequence-specific RNA-binding proteins for organellar RNAs in plants.},
  author={Takahiro J. Nakamura and Yusuke Yagi and Keiko Kobayashi},
  journal={Plant \& cell physiology},
  year={2012},
  volume={53 7},
  pages={
          1171-9
        }
}
The pentatricopeptide repeat (PPR) protein family is highly expanded in terrestrial plants. Arabidopsis contains 450 PPR genes, which represents 2% of the total protein-coding genes. PPR proteins are eukaryote-specific RNA-binding proteins implicated in multiple aspects of RNA metabolism of organellar genes. Most PPR proteins affect a single or small subset of gene(s), acting in a gene-specific manner. Studies over the last 10 years have revealed the significance of this protein family in… 
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The next challenge will be to refine the understanding of the function of the proteins and to resolve the yeast PPR-RNA-binding code, which might differ significantly from the plant PPR code.
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Amino acid variation at 3 particular positions within the PPR motif determined recognition of a specific RNA in a programmable manner, with a 1-motif to 1-nucleotide correspondence, with no gap sequence.
Functional analysis of the Arabidopsis thaliana CHLOROPLAST BIOGENESIS 19 pentatricopeptide repeat editing protein.
TLDR
Using biomolecular fluorescence complementation and site-directed mutagenesis, it is demonstrated that the E domain of CLB19 interacts with the RNA-interacting protein MORF2/RIP2 but not with MORF9/RIP9, supporting an important role of plastids in female gametogenesis.
Recent Progress Toward RNA Manipulation with Engineered Pentatricopeptide Repeat Proteins
TLDR
The engineered PPR motifs fused with various effector domains are shown to bind to and manipulate RNAs in a controlled manner and the potentials of PPR as novel, versatile tools for RNA manipulation are discussed.
The conserved domain in MORF proteins has distinct affinities to the PPR and E elements in PPR RNA editing factors.
The rice pentatricopeptide repeat protein PPR756 is involved in pollen development by affecting multiple RNA editing in mitochondria
TLDR
A role is established for PPR756 in rice development, participating in RNA editing of three various transcripts and cooperating with OsMORFs via an editosome manner in rice.
The Rice Pentatricopeptide Repeat Protein PPR756 Is Involved in Pollen Development by Affecting Multiple RNA Editing in Mitochondria
TLDR
A role is established for PPR756 in rice development, participating in RNA editing of three various transcripts and cooperating with OsMORFs via an editosome manner in rice.
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