Mechanistic aspects of cyanogenesis from active-site mutant Ser80Ala of hydroxynitrile lyase from Manihot esculenta in complex with acetone cyanohydrin.

Abstract

The structure and function of hydroxynitrile lyase from Manihot esculenta (MeHNL) have been analyzed by X-ray crystallography and site-directed mutagenesis. The crystal structure of the MeHNL-S80A mutant enzyme has been refined to an R-factor of 18.0% against diffraction data to 2.1-A resolution. The three-dimensional structure of the MeHNL-S80A-acetone… (More)

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@article{Lauble2001MechanisticAO, title={Mechanistic aspects of cyanogenesis from active-site mutant Ser80Ala of hydroxynitrile lyase from Manihot esculenta in complex with acetone cyanohydrin.}, author={Hanspeter Lauble and Burkhard Miehlich and Siegfried F{\"{o}rster and Harald Wajant and Franz Effenberger}, journal={Protein science : a publication of the Protein Society}, year={2001}, volume={10 5}, pages={1015-22} }