Mechanistic analysis of wheat chlorophyllase.

@article{Arkus2005MechanisticAO,
  title={Mechanistic analysis of wheat chlorophyllase.},
  author={Kiani A J Arkus and Edgar B Cahoon and Joseph M Jez},
  journal={Archives of biochemistry and biophysics},
  year={2005},
  volume={438 2},
  pages={146-55}
}
Chlorophyllase catalyzes the initial step in the degradation of chlorophyll and plays a key role in leaf senescence and fruit ripening. Here, we report the cloning of chlorophyllase from Triticum aestivum (wheat) and provide a detailed mechanistic analysis of the enzyme. Purification of recombinant chlorophyllase from an Escherichia coli expression system indicates that the enzyme functions as a dimeric protein. Wheat chlorophyllase hydrolyzed the phytol moiety from chlorophyll (k(cat) = 566… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 19 extracted citations

Similar Papers

Loading similar papers…