Mechanistic analysis of wheat chlorophyllase.

  title={Mechanistic analysis of wheat chlorophyllase.},
  author={Kiani A J Arkus and Edgar B Cahoon and Joseph M Jez},
  journal={Archives of biochemistry and biophysics},
  volume={438 2},
Chlorophyllase catalyzes the initial step in the degradation of chlorophyll and plays a key role in leaf senescence and fruit ripening. Here, we report the cloning of chlorophyllase from Triticum aestivum (wheat) and provide a detailed mechanistic analysis of the enzyme. Purification of recombinant chlorophyllase from an Escherichia coli expression system indicates that the enzyme functions as a dimeric protein. Wheat chlorophyllase hydrolyzed the phytol moiety from chlorophyll (k(cat) = 566… CONTINUE READING


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