Mechanisms subserving the steroidogenic synergism between follicle-stimulating hormone and insulin-like growth factor I (somatomedin C). Alterations in cellular sterol metabolism in swine granulosa cells.

@article{Veldhuis1987MechanismsST,
  title={Mechanisms subserving the steroidogenic synergism between follicle-stimulating hormone and insulin-like growth factor I (somatomedin C). Alterations in cellular sterol metabolism in swine granulosa cells.},
  author={Johannes D. Veldhuis and Raymond J Rodgers},
  journal={The Journal of biological chemistry},
  year={1987},
  volume={262 16},
  pages={
          7658-64
        }
}
Regulation of Porcine Granulosa Cell Steroidogenic Acute Regulatory Protein (StAR) by Insulin-Like Growth Factor I: Synergism with Follicle-Stimulating Hormone or Protein Kinase A Agonist1.
TLDR
This work generated a partial porcine StAR complementary DNA by RT-PCR and used the corresponding antisense riboprobe to quantify the control of StAR gene expression by FSH and insulin-like growth factor I in hormonally responsive swine granulosa cells, which typically manifest synergistic steroidogenic stimulation by these two dominant intrafollicular regulators.
2-hydroxyestradiol enhanced progesterone production by porcine granulosa cells: dependence on de novo cholesterol synthesis and stimulation of cholesterol side-chain cleavage activity and cytochrome P450scc messenger ribonucleic acid levels.
TLDR
The hypothesis that 2-OH-E2-enhanced progesterone biosynthesis by porcine granulosa cells is dependent on de novo cholesterol synthesis and is associated with increased levels of the mRNA encoding cytochrome P-450scc, which leads to increases in basal and gonadotropin-induced SCC activity is supported.
Regulatory Actions of the Insulin-Like Growth Factor, IGF-I (Somatomedin-C), on Sterol Metabolism by Ovarian Cells
TLDR
An in vitro serum-free culture system of swine granulosa cells is employed that is highly responsive to the insulin-like growth factor, IGF-I (Somatomedin-C), and the mechanisms subserving the actions of differentiating growth factors are examined.
Insulin-Like Growth Factor-I Selectively Stimulates Cholesterol Side-Chain Cleavage Expression in Ovarian Theca-lnterstitial Cells
TLDR
The purpose of the present studies was to begin to elucidate the molecular mechanisms of the interaction between IGF-I and LH.
Mechanisms of Insulin-Like Growth Factor I Augmentation of Follicle-Stimulating Hormone-Induced Porcine Steroidogenic Acute Regulatory Protein Gene Promoter Activity in Granulosa Cells* *This work was supported by the Lalor Foundation, NIH National Research Service Awards Grants HD-07382 and HD-0801
TLDR
Investigation of potential mechanisms of IGF-I synergy with FSH and the protein kinase A (PKA) pathway in activating the porcine StAR gene promoter finds 5′-deletional constructs coupled to a cytoplasmically targeted firefly luciferase reporter gene.
Follicle-stimulating hormone regulates low density lipoprotein metabolism by swine granulosa cells.
TLDR
It is concluded that FSH and LDL synergistically enhance progesterone biosynthesis by swine granulosa cells via cellular mechanisms that result in significantly augmented binding, internalization, and degradation of LDL in concert with increased effectual utilization of sterol substrate in the cholesterol side-chain cleavage reaction.
Insulin-like growth factor type I increases concentrations of messenger ribonucleic acid encoding cytochrome P450 cholesterol side-chain cleavage enzyme in primary cultures of porcine granulosa cells.
TLDR
Induction of primary cultures of immature porcine granulosa cells with IGF-I will increase progesterone production as well as the synthesis of immunoprecipitable P450scc enzyme, and possible molecular mechanisms subserving these inductive effects of IGF- I were examined.
Concerted Regulation of Low Density Lipoprotein Receptor Gene Expression by Follicle-Stimulating Hormone and Insulin-Like Growth Factor I in Porcine Granulosa Cells: Promoter Activation, Messenger Ribonucleic Acid Stability, and Sterol Feedback* *This work was supported by the Lalor Foundation, NIH
TLDR
The combination of FSH and IGF-I can induce accumulation of LDL receptor mRNA in cultured granulosa cells even in the presence of sterol negative feedback and can do so mechanistically by a combination of promoter activation and increased mRNA stability.
Different action of ovine GH on porcine theca and granulosa cells proliferation and insulin-like growth factors I- and II-stimulated estradiol production.
TLDR
Differences in the estradiol production in response to GH alone and the effect of the synergistic action of GH and IGFs suggest that different cellular mechanisms for these hormones are triggered in each cell type.
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