Mechanisms of unintended amino acid sequence changes in recombinant monoclonal antibodies expressed in Chinese Hamster Ovary (CHO) cells.

@article{Guo2010MechanismsOU,
  title={Mechanisms of unintended amino acid sequence changes in recombinant monoclonal antibodies expressed in Chinese Hamster Ovary (CHO) cells.},
  author={Donglin Guo and Albert Gao and David A. Michels and Lauren Feeney and Marian Eng and Betty Chan and Michael W Laird and Boyan Zhang and X. Christopher Yu and John C Joly and Brad Snedecor and Amy Shen},
  journal={Biotechnology and bioengineering},
  year={2010},
  volume={107 1},
  pages={163-71}
}
An amino acid sequence variant is defined as an unintended amino acid sequence change and contributes to product heterogeneity. Recombinant monoclonal antibodies (MAbs) are primarily expressed from Chinese Hamster Ovary (CHO) cells using stably transfected production cell lines. Selections and amplifications with reagents such as methotrexate (MTX) are often required to achieve high producing stable cell lines. Since MTX is often used to generate high producing cell lines, we investigated the… CONTINUE READING
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