Mechanisms of oxidant-mediated cell injury. The glycolytic and mitochondrial pathways of ADP phosphorylation are major intracellular targets inactivated by hydrogen peroxide.

@article{Hyslop1988MechanismsOO,
  title={Mechanisms of oxidant-mediated cell injury. The glycolytic and mitochondrial pathways of ADP phosphorylation are major intracellular targets inactivated by hydrogen peroxide.},
  author={Paul A Hyslop and Daniel B Hinshaw and W A Halsey and Ingrid U. Schraufst{\"a}tter and Richard D. Sauerheber and Roger G. Spragg and Janis Harline Jackson and Charles G. Cochrane},
  journal={The Journal of biological chemistry},
  year={1988},
  volume={263 4},
  pages={1665-75}
}
Inhibition of ADP phosphorylation by both glycolysis and mitochondria in P388D1 cells exposed to H2O2 is described. Net glucose uptake and lactate production were inhibited by oxidant exposure (ED50 = 50-100 microM). Glycolysis was specifically inactivated at the glyceraldehyde-3-phosphate dehydrogenase step by three independent mechanisms: (a) direct inactivation of the intracellular enzyme (ED50 approximately equal to 100 microM); (b) reduction of the intracellular concentration and redox… CONTINUE READING