Mechanisms of intracellular protein transport

  title={Mechanisms of intracellular protein transport},
  author={James E. Rothman},
  • J. Rothman
  • Published 3 November 1994
  • Biology, Chemistry
  • Nature
Recent advances have uncovered the general protein apparatus used by all eukaryotes for intracellular transport, including secretion and endocytosis, and for triggered exocytosis of hormones and neurotransmitters. Membranes are shaped into vesicles by cytoplasmic coats which then dissociate upon GTP hydrolysis. Both vesicles and their acceptor membranes carry targeting proteins which interact specifically to initiate docking. A general apparatus then assembles at the docking site and fuses the… 

Protein Sorting by Transport Vesicles

Eukaryotic life depends on the spatial and temporal organization of cellular membrane systems and general principles that underlie a broad variety of physiological processes, including cell surface growth, the biogenesis of distinct intracellular organelles, endocytosis, and the controlled release of hormones and neurotransmitters.

Felix Hoppe-Seyler Lecture 1996. Mechanisms of intracellular protein transport.

Transport vesicles are formed as coat proteins assemble on membranes, are targetted by SNARE proteins, and fuse using a general mechanism involving SNAP proteins and the ATPase NSF.

Protein-protein interactions in intracellular membrane fusion.

The Mechanisms of Vesicle Budding and Fusion

Vesicular transport: the core machinery of COPI recruitment and budding.

This work has generated a model that covers the cycle of coat recruitment, coat polymerization, vesicle budding and uncoating in COPI vesicles, and shed light on the mechanism of COPIvesicle biogenesis.

Vesicular protein transport

  • P. Schu
  • Biology
    The Pharmacogenomics Journal
  • 2001
This review describes the individual events involved in the process of vesicle mediated protein transport and sorting and summarizes the knowledge about the function of proteins and lipids orchestrating the process.

Rab proteins as membrane organizers

Cellular organelles in the exocytic and endocytic pathways have a distinctive spatial distribution and communicate through an elaborate system of vesiculo-tubular transport. Rab proteins and their

SNAREs and the specificity of transport vesicle targeting.

  • M. Bennett
  • Biology, Chemistry
    Current opinion in cell biology
  • 1995



Protein-protein interactions contributing to the specificity of intracellular vesicular trafficking.

A physical basis for the specificity of intracellular vesicle transport is suggested by the demonstration of specific interaction between vesicular proteins and plasma membrane proteins.

Vesicle fusion following receptor-mediated endocytosis requires a protein active in Golgi transport

It is shown that the same N-ethylmaleimide-sensitive factor that catalyses the vesicle-mediated transport within Golgi stacks is also required for endocytic vesicles fusion, suggesting that a common mechanism for vESicle fusion exists for both the secretory and endocytical pathways of eukaryotic cells.

The molecular machinery for secretion is conserved from yeast to neurons.

  • M. BennettR. Scheller
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1993
The results that support this possibility and a model for synaptic vesicle docking and fusion that incorporates evolutionarily conserved elements that may be part of a constitutive fusion machinery and specialized elements that might mediate regulatory events that are specific to the process of neurotransmitter release are discussed.

A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast

A protein sensitive toN-ethylmaleimide catalyses the fusion of transport vesicles with Golgi cisternae in a mammalian cell-free system and is equivalent to the SEC18 gene product of the yeast Saccharomyces cerevisiae, known to be essential for vesicle-mediated transport from the endoplasmic reticulum to the Golgi apparatus.

Mediation of the attachment or fusion step in vesicular transport by the GTP-binding Ypt1 protein

A specific GTP-binding protein is required for either attachment or fusion (or both) of secretory vesicles with the acceptor compartment during protein secretion.