Mechanisms of excitation-contraction coupling failure during metabolic inhibition in guinea-pig ventricular myocytes.

@article{Goldhaber1991MechanismsOE,
  title={Mechanisms of excitation-contraction coupling failure during metabolic inhibition in guinea-pig ventricular myocytes.},
  author={Joshua I. Goldhaber and James M Parker and James N. Weiss},
  journal={The Journal of physiology},
  year={1991},
  volume={443},
  pages={371-86}
}
1. The effects of complete metabolic inhibition on excitation-contraction coupling in heart were studied by exposing patch-clamped guinea-pig ventricular myocytes, loaded via the patch pipette with the Ca2+ indicator Fura-2 (0.1 mM), to carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP, 1 microM) and 2-deoxyglucose (2-DG, 10 mM) while simultaneously… CONTINUE READING