Mechanisms of endocytosis.

@article{Doherty2009MechanismsOE,
  title={Mechanisms of endocytosis.},
  author={Gary J. Doherty and Harvey T. McMahon},
  journal={Annual review of biochemistry},
  year={2009},
  volume={78},
  pages={
          857-902
        }
}
Endocytic mechanisms control the lipid and protein composition of the plasma membrane, thereby regulating how cells interact with their environments. Here, we review what is known about mammalian endocytic mechanisms, with focus on the cellular proteins that control these events. We discuss the well-studied clathrin-mediated endocytic mechanisms and dissect endocytic pathways that proceed independently of clathrin. These clathrin-independent pathways include the CLIC/GEEC endocytic pathway… 

Figures and Tables from this paper

Clathrin-independent pathways of endocytosis.

TLDR
This review explores pathways of endocytosis that occur in the absence of clathrin that are likely to play key roles in the regulation of plasma membrane area and tension and also control the availability of membrane during cell migration.

Endocytic vacuole formation by WASH-mediated endocytosis

TLDR
The results indicate cargo uptake by uncoated, inward-budding pits facilitated by the membrane bending retromer protein SNX2 is promoted by WASH, an actin regulator typically not found at the plasma membrane.

Trafficking pathway between plasma membrane and mitochondria via clathrin-mediated endocytosis

TLDR
It is reported that FM dyes, membrane-impermeant fluorescent lipid probes, can traffic to mitochondria directly from the plasma membrane by clathrin-mediated endocytosis.

[Clathrin-independent endocytosis - role in disease processes and pharmaceutical aspects].

TLDR
Diseases associated with genetic defects of proteins involved in CIE are characterized and the relationship between expression of these proteins and pathology of atherosclerosis, hypercholesterolemia, diabetes and neoplasia is presented.

The mammalian endocytic cytoskeleton.

Visualizing the functional architecture of the endocytic machinery

TLDR
A fluorescence microscopy method is developed to track the average positions of yeast endocytic proteins in relation to each other with a time precision below 1 s and with a spatial precision of ∼10 nm, which shows how different coat proteins are distributed within the coat structure and how the assembly dynamics of N-BAR proteins relate to membrane shape changes.

Spoiled for Choice: Diverse Endocytic Pathways Function at the Cell Surface.

TLDR
A current survey of the different types of endocytic pathways available at the cell surface is provided and a new classification and plausible molecular mechanisms for some of the less characterized pathways are discussed.

Local actin polymerization during endocytic carrier formation.

TLDR
The understanding of the molecular steps mediating local actin polymerization during the formation of endocytic carriers is reviewed to clarify the roles of RhoA and formin-mediated actin bundling during endocytosis.
...

References

SHOWING 1-10 OF 332 REFERENCES

ARF1 is directly involved in dynamin-independent endocytosis

TLDR
It is shown that reduction in activity or levels of ARF1 specifically inhibits GPI-AP and fluid-phase endocytosis without affecting other clathrin-dependent or independent endocytic pathways.

Genome-wide analysis of human kinases in clathrin- and caveolae/raft-mediated endocytosis

TLDR
It is shown that a high number of kinases are involved in endocytosis, and that each endocytic route is regulated by a specific kinase subset, suggesting coordinate regulation.

Flotillin-1 defines a clathrin-independent endocytic pathway in mammalian cells

TLDR
It is proposed that flotillin-1 is one determinant of a clathrin-independent endocytic pathway in mammalian cells and resides in punctate structures within the plasma membrane and in a specific population of endocytosis intermediates.

A distinct class of endosome mediates clathrin-independent endocytosis to the Golgi complex

TLDR
Show that molecules which partition into DRMs and are endocytosed in a clathrin-independent fashion, accumulate in a discrete population of endosomes en route to the Golgi complex, which is devoid of markers for classical early and recycling endosome, but do contain caveolin-1.

Assembly of endocytic machinery around individual influenza viruses during viral entry

TLDR
This work has studied the entry mechanisms of influenza viruses by tracking the interaction of single viruses with cellular endocytic structures in real time using fluorescence microscopy, and shows that influenza can exploit clathrin-mediated and clathin- and caveolin-independent endocytical pathways in parallel, both pathways leading to viral fusion with similar efficiency.

Internalization of large double-membrane intercellular vesicles by a clathrin-dependent endocytic process.

TLDR
It is reported that entire GJ plaques, clusters of GJ channels, can be internalized to form large, double-membrane vesicles previously termed annular gap junctions (AGJs), which contribute to the understanding of clathrin's numerous emerging functions.

Actin in the endocytic pathway: From yeast to mammals

...