Mechanisms of Surface-Mediated DNA Hybridization

  title={Mechanisms of Surface-Mediated DNA Hybridization},
  author={Jon H. Monserud and Daniel K. Schwartz},
  booktitle={ACS nano},
Single-molecule total internal reflection fluorescence microscopy was employed in conjunction with resonance energy transfer (RET) to observe the dynamic behavior of donor-labeled ssDNA at the interface between aqueous solution and a solid surface decorated with complementary acceptor-labeled ssDNA. At least 100,000 molecular trajectories were determined for both complementary strands and negative control ssDNA. RET was used to identify trajectory segments corresponding to the hybridized state… CONTINUE READING
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