Corpus ID: 83277543

Mechanisms of Protease Action

  title={Mechanisms of Protease Action},
  author={L. Polgár},
The Chemistry of the Peptide Bond. General Aspects of Proteases. Serine Proteases. Cysteine Proteases. Aspartic Proteases. Metalloproteases. Index. 
Silica-based artificial protease exploiting aldehyde groups as catalytic elements.
An artificial protease synthesized by covering the surface of silica gel with aldehyde and indole groups effectively hydrolyzed albumin and gamma-globulin. It is proposed that the aldehyde group isExpand
Potential irreversible inhibitors of cysteine proteases based on sultam and naphthoquinone scaffolds
Tese de doutoramento em Farmacia (Quimica Farmaceutica), apresentada a Universidade de Lisboa atraves da Faculdade de Farmacia, 2008
Papain-like proteases: Applications of their inhibitors
This review hopes to bring together the information available about the proteases with particular emphasis on papain-like plant cysteine proteases and their potential utilities. Expand
Characterization of conjugated protease inhibitors
Billinger, E. 2020. Characterization of conjugated protease inhibitors. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 1888. 86 pp. Uppsala: ActaExpand
Protease Specificity and Protein Sequence Analysis
Specificity is explained in terms of the second-order rate constant of the reaction between the free enzyme and its substrate. Depending on the different characteristics of the substrate, severalExpand
Prolyl Oligopeptidase An Unusual β-Propeller Domain Regulates Proteolysis
A resolution crystal structure of prolyl oligopeptidase, a large cytosolic enzyme that belongs to a new class of serine peptidases, is presented, which may facilitate drug design to treat memory disorders. Expand
Protease-catalyzed hydrolysis of substrate mimetics (inverse substrates): A new approach reveals a new mechanism.
The experimental and theoretical results obtained for the serine protease trypsin suggest a novel extended kinetic model which explains the hydrolysis of these types of protease substrates and accounts for the structural consequences for their aminolysis. Expand
Novel enzymatic activity derived from the Semliki Forest virus capsid protein.
The data provide further evidence for the potential catalytic activity of natively unfolded proteins, and provide the basis for engineering of alphavirus capsid proteins towards hydrolytic enzymes with novel specificities. Expand
Basic Kinetic Mechanisms of Proteolytic Enzymes
Peptidases, like other enzymes, generally follow Michaelis—Menten kinetics, and their reactions can be characterized by kcat, Km and particularly kcat/Km. Each of these parameters may involve severalExpand
Studies on the Catalytic Mechanism of a Glutamic Peptidase*
The solvent kinetic isotope effects and the proton inventory experiments support a mechanism for the glutamic peptidase SGP that involves the nucleophilic attack of the general base (Glu136) activated water, and establish a fundamental role of the S1 subsite interactions in promoting catalysis. Expand