Mechanisms of H+ secretion by inner medullary collecting duct cells.

  title={Mechanisms of H+ secretion by inner medullary collecting duct cells.},
  author={A M Selvaggio and John H. Schwartz and H. H. Bengele and Fredric David Gordon and Emily A Alexander},
  journal={The American journal of physiology},
  volume={254 3 Pt 2},
Inner medullary collecting duct cells were isolated from rat papillae and grown to confluence on cover slips. H+ secretion was estimated by intracellular pH (pHi) changes measured with the fluorescent probe 2,7-biscarboxyethyl-5(6)-carboxyfluorescein. In buffered NaCl, pHi was 7.14 +/- 0.04 (n = 78). After acidification about 40% of monolayers exhibited Na+-independent alkalinization. In 5 mM glucose, cell alkalinization occurred at a rate of 47 +/- 4 nM H+/min. However, cell alkalinization did… CONTINUE READING

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