Mechanisms of Barbiturate Inhibition of Acetylcholine Receptor Channels

@article{Dilger1997MechanismsOB,
  title={Mechanisms of Barbiturate Inhibition of Acetylcholine  Receptor Channels },
  author={James P. Dilger and Rebecca Boguslavsky and Martin Barann and Tamir Katz and Ana Maria Vidal},
  journal={The Journal of General Physiology},
  year={1997},
  volume={109},
  pages={401 - 414}
}
We used patch clamp techniques to study the inhibitory effects of pentobarbital and barbital on nicotinic acetylcholine receptor channels from BC3H-1 cells. Single channel recording from outside-out patches reveals that both drugs cause acetylcholine-activated channel events to occur in bursts. The mean duration of gaps within bursts in 2 ms for 0.1 mM pentobarbital and 0.05 ms for 1 mM barbital. In addition, 1 mM barbital reduces the apparent single channel current by 15%. Both barbiturates… CONTINUE READING