Mechanisms for bacterial gliding motility on soft substrates

@article{Tchoufag2019MechanismsFB,
  title={Mechanisms for bacterial gliding motility on soft substrates},
  author={Jo{\"e}l Tchoufag and Pushpita Ghosh and Connor B. Pogue and Beiyan Nan and Kranthi K. Mandadapu},
  journal={Proceedings of the National Academy of Sciences},
  year={2019},
  volume={116},
  pages={25087 - 25096}
}
Significance Gliding motility is the ability of certain rod-shaped bacteria to translocate on surfaces without the aid of external appendages such as flagella, cilia, or pili. This motility is crucial to their developmental cycle because it regulates their proliferation in the presence of nutrients or aggregation to form fruiting bodies in starvation conditions. Using myxobacteria as a canonical example of these organisms, we show that single-cell gliding is mediated by elastic, viscous, and… 

Figures from this paper

A computational approach to model gliding motion of an organism on a sticky slime layer over a solid substrate

To simulate the locomotion of such gliding microorganisms, a wavy sheet over Oldroyd-4 constant fluid is utilized and the equations regulating the flow of slime beneath the cell/organism are developed.

Three-Dimensional Observations of an Aperiodic Oscillatory Gliding Behavior in Myxococcus xanthus Using Confocal Interference Reflection Microscopy

The first use of confocal multiwavelength interference reflection microscopy (IRM) to study gliding bacteria is presented, revealing aperiodic oscillatory behavior with changes in the position of the basal membrane relative to the substrate on the order of 90 nm in vitro.

Structural mechanics of filamentous cyanobacteria

This work quantifies the bending stiffness of three species of filamentous cyanobacteria, of order Oscillatoriales, using a microfluidic flow device where single filaments are deflected by fluid flow, and finds that the stiffness of the cyanob bacteria is well-captured by a simple model of a flexible rod.

A comprehensive numerical investigation of Carreau-Yasuda slime beneath complex bacterial wavy surface

: Gliding motility often noticed in phylogenetically rod-shaped bacteria which locomote via dissipating their own energy. The said gliding motion via producing waves and secreting slime is generally

Methods to Evaluate Bacterial Motility and Its Role in Bacterial–Host Interactions

The most common techniques and recent advances are presented and their strengths as well as their limitations are discussed and the advantages of three-dimensional imaging in microscopic approaches are highlighted.

Cluster and conquer: the morphodynamics of invasion of a compliant substrate by active rods.

Furrow networks created by the active particles have a fractal-like structure whose dimension varies systematically with substrate stiffness but is less sensitive to particle activity, suggesting that, to sustain such extensive furrow networks, colonies must regulate their overall growth rate.

Chemotactic Bacteria Facilitate the Dispersion of Nonmotile Bacteria through Micrometer-Sized Pores in Engineered Porous Media

Chemotaxis-mediated cotransport of bacterial degraders and its implications in pore accessibility opens new avenues for the enhancement of bacterial dispersion in porous media and the biodegradation of heterogeneously contaminated scenarios.

Pectin Induced Colony Expansion of Soil-Derived Flavobacterium Strains

It is suggested that pectin may facilitate flavob bacterial expansion on plant surfaces in addition to serving as an essential carbon source within the context of flavobacterial-plant interactions.

References

SHOWING 1-10 OF 95 REFERENCES

Bacterial gliding motility: multiple mechanisms for cell movement over surfaces.

  • M. McBride
  • Biology
    Annual review of microbiology
  • 2001
Genetic, biochemical, ultrastructural, and behavioral studies are providing insight into the machineries employed by these diverse bacteria that enable them to glide over surfaces.

Novel mechanisms power bacterial gliding motility

Recent findings made on the gliding mechanisms of the myxobacteria, flavobacteria and mycoplasmas are summarized to provide rich source materials for studying the function and evolution of complex microbial nanomachines.

Uncovering the mystery of gliding motility in the myxobacteria.

Recent studies suggest that gliding motility in M. xanthus involves large multiprotein structural complexes, regulatory proteins, and cytoskeletal filaments, and alternative models for gliding are discussed.

Myxobacteria gliding motility requires cytoskeleton rotation powered by proton motive force

Evidence is presented that periplasmic AgmU decorates a looped continuous helix that rotates clockwise as cells glide forward, reversing its rotation when cells reverse polarity, which is consistent with a mechanochemical model in which PMF-driven motors run along an endless looped helical track.

MotAB-like machinery drives the movement of MreB filaments during bacterial gliding motility

It is found that a subpopulation of MreB particles moves rapidly along helical trajectories, similar to the movements of the MotAB-like gliding motors, and this rapid movement was not affected by the inhibitors of cell wall biosynthesis.

Flagella stator homologs function as motors for myxobacterial gliding motility by moving in helical trajectories

This work shows that the untethered gliding motors of M. xanthus, by moving within the membrane, can transform helical motion into linear driving forces that push against the surface.

The mechanism of force transmission at bacterial focal adhesion complexes

It is shown that the Agl–Glt machinery contains an inner-membrane motor complex that moves intracellularly along a right-handed helical path; when the machinery becomes stationary at FASs, the motor complex powers a left-handed rotation of the cell around its long axis.

Gliding motility and polarized slime secretion

It is proposed that transposon insertions in glycosyltransferase genes carry out the synthesis of a repeat unit polysaccharide that constitutes the slime.

Evidence That Focal Adhesion Complexes Power Bacterial Gliding Motility

The dynamics of protein cluster localization suggest that intracellular motors and force transmission by dynamic focal adhesions can power bacterial motility.

Myxococcus xanthus Gliding Motors Are Elastically Coupled to the Substrate as Predicted by the Focal Adhesion Model of Gliding Motility

Using a biophysical model of the M. xanthus cell, evidence is found for elastic coupling in support of the focal adhesion model and comparison of modeling results with experimental data for cell-cell collision events pointed to a strong, elastic attachment.
...