Mechanisms for GroEL/GroES-mediated folding of a large 86-kDa fusion polypeptide in vitro.

@article{Huang1999MechanismsFG,
  title={Mechanisms for GroEL/GroES-mediated folding of a large 86-kDa fusion polypeptide in vitro.},
  author={Yihua Huang and David T Chuang},
  journal={The Journal of biological chemistry},
  year={1999},
  volume={274 15},
  pages={
          10405-12
        }
}
Our understanding of mechanisms for GroEL/GroES-assisted protein folding to date has been derived mostly from studies with small proteins. Little is known concerning the interaction of these chaperonins with large multidomain polypeptides during folding. In the present study, we investigated chaperonin-dependent folding of a large 86-kDa fusion polypeptide, in which the mature maltose-binding protein (MBP) sequence was linked to the N terminus of the alpha subunit of the decarboxylase (E1… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 11 extracted citations