Mechanism of the intramolecular Claisen condensation reaction catalyzed by MenB, a crotonase superfamily member.

@article{Li2011MechanismOT,
  title={Mechanism of the intramolecular Claisen condensation reaction catalyzed by MenB, a crotonase superfamily member.},
  author={Huei-Jiun Li and Xiaokai Li and Nina Liu and Huaning Zhang and James J Truglio and Shambhavi Mishra and Caroline Kisker and Miguel Garc{\'i}a-D{\'i}az and Peter J. Tonge},
  journal={Biochemistry},
  year={2011},
  volume={50 44},
  pages={9532-44}
}
MenB, the 1,4-dihydroxy-2-naphthoyl-CoA synthase from the bacterial menaquinone biosynthesis pathway, catalyzes an intramolecular Claisen condensation (Dieckmann reaction) in which the electrophile is an unactivated carboxylic acid. Mechanistic studies on this crotonase family member have been hindered by partial active site disorder in existing MenB X-ray structures. In the current work the 2.0 Å structure of O-succinylbenzoyl-aminoCoA (OSB-NCoA) bound to the MenB from Escherichia coli… CONTINUE READING