Mechanism of the EPSP synthase catalyzed reaction: evidence for the lack of a covalent carboxyvinyl intermediate in catalysis.

Abstract

In order to detect covalent reaction intermediates in the 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase reaction, we have investigated the interaction of EPSP synthase with the reaction product EPSP. An exchange of EPSP-methylene protons could be demonstrated by incubating EPSPS with EPSP in D2O. Since trace amounts of contaminating Pi would lead to… (More)

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Cite this paper

@article{Wibbenmeyer1988MechanismOT, title={Mechanism of the EPSP synthase catalyzed reaction: evidence for the lack of a covalent carboxyvinyl intermediate in catalysis.}, author={Jamie A. Wibbenmeyer and L A Brundage and Stephen R. Padgette and John J Likos and Garima Kishore}, journal={Biochemical and biophysical research communications}, year={1988}, volume={153 2}, pages={760-6} }