Mechanism of rise and decay of thapsigargin-evoked calcium signals in MDCK cells.

@article{Jan1998MechanismOR,
  title={Mechanism of rise and decay of thapsigargin-evoked calcium signals in MDCK cells.},
  author={C. Ruppert Jan and Christoph M. Ho and Sheng-Nan Wu and C. J. Tseng},
  journal={Life sciences},
  year={1998},
  volume={64 4},
  pages={
          259-67
        }
}
We studied the effect of thapsigargin on intracellular calcium levels ([Ca2+]i) measured by fura-2 fluorimetry in Madin Darby canine kidney (MDCK) cells. Thapsigargin elevated [Ca2+]i dose dependently with an EC50 of approximately 0.15 microM. The Ca2+ signal consisted of a slow rise, a gradual decay and a plateau. Depletion of the endoplasmic reticulum Ca2+ store with thapsigargin for 7 min abolished the [Ca2+]i increases evoked by bradykinin. Removal of extracellular Ca2+ reduced the… CONTINUE READING
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