Mechanism of plasmid delivery by hydrodynamic tail vein injection. I. Hepatocyte uptake of various molecules

@article{Sebestyn2006MechanismOP,
  title={Mechanism of plasmid delivery by hydrodynamic tail vein injection. I. Hepatocyte uptake of various molecules},
  author={Magdolna G. Sebesty{\'e}n and Vladimir G. Budker and Tatiana Budker and Vladimir M. Subbotin and Guofeng Zhang and Sean D. Monahan and David L. Lewis and So C Wong and James E. Hagstrom and J. A. Wolff},
  journal={The Journal of Gene Medicine},
  year={2006},
  volume={8}
}
The hydrodynamic tail vein (HTV) injection of naked plasmid DNA is a simple yet effective in vivo gene delivery method into hepatocytes. It is increasingly being used as a research tool to elucidate mechanisms of gene expression and the role of genes and their cognate proteins in the pathogenesis of disease in animal models. A greater understanding of its mechanism will aid these efforts and has relevance to macromolecular and nucleic acid delivery in general. 

Mechanism of plasmid delivery by hydrodynamic tail vein injection. II. Morphological studies

The goal was to study the uptake mechanism by following the morphological changes in the liver and correlate these with the fate of the injected plasmid DNA.

Gene therapy progress and prospects: Hydrodynamic gene delivery

The recent development of the hydrodynamic limb vein procedure provides a safe nucleic acid delivery technique with equally high efficiency in small and large research animals and, importantly, the prospects for clinical translation.

Hydrodynamic gene delivery: its principles and applications.

  • T. SudaDexi Liu
  • Biology
    Molecular therapy : the journal of the American Society of Gene Therapy
  • 2007
This review provides an overview of the theory and practice of hydrodynamic gene delivery so as to aid researchers for the use of this method in their pre-clinical and translational gene therapy studies.

DNA delivery to ‘ex vivo’ human liver segments

It is shown that retrograde venous injection of watertight human liver segment is an anadromous procedure that results in wide liver gene delivery and good gene expression, and additional studies will be necessary to clarify the influence of the prolonged ischemia injury to hepatocytes in this model.

Preferential delivery of the Sleeping Beauty transposon system to livers of mice by hydrodynamic injection

The hydrodynamic delivery procedure for the SB transposon system that allows efficient delivery to the liver in the mouse and results in expression of the transgene in 5–40% of hepatocytes 1 d after injection.

Animal Models for Hydrodynamic Gene Delivery

The aim of this chapter is to describe the basic principles of the hydrodynamic gene delivery, the surgical procedures in all animal models and the reflection of the scope for the future development.

Low RNA translation activit limits the efficacy of hydrodynamic gene transfer to pig liver in vivo

Hydrodynamic gene delivery has proved an efficient strategy for nonviral gene therapy in the murine liver but it has been less efficient in pigs, so a surgical strategy to seal the whole pig liver in vivo is used.

Liver-targeted gene therapy by SV40-based vectors using the hydrodynamic injection method.

The SV40 vector proved to be efficient in gene delivery to the liver, leading to long-term (> or =107 days) transgene expression in hepatocytes, suggesting that SV40 vectors may be useful for liver gene therapy.

Hydrodynamic delivery of siRNA in a mouse model of sepsis.

The use of hydrodynamic administration as a technique to deliver, in vivo, naked siRNA constructs into experimental animals as a method of transient gene knockdown may prove useful in situations where knockout animals do not exist, or to determine the effect of gene Knockdown at specific time points during an experiment.
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References

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Mechanism of plasmid delivery by hydrodynamic tail vein injection. II. Morphological studies

The goal was to study the uptake mechanism by following the morphological changes in the liver and correlate these with the fate of the injected plasmid DNA.

Uptake by mouse liver and intracellular fate of plasmid DNA after a rapid tail vein injection of a small or a large volume

An efficient gene transfer can be achieved in mouse liver by a rapid tail vein injection of a large volume of plasmid DNA solution (hydrodynamics‐based transfection) and it must be related to the uptake and intracellular fate of DNA.

Hydrodynamic delivery of DNA

The development of the procedures for hydrodynamic delivery will be described and the parameters necessary for attaining maximal gene expression will be highlighted, as well as potential uses of this approach in various research and clinical applications.

Hydroporation as the mechanism of hydrodynamic delivery

The hydrodynamic injection induces a transient irregularity of heart function, a sharp increase in venous pressure, an enlargement of liver fenestrae, and enhancement of membrane permeability of the hepatocytes, suggesting that hepatic delivery by the hydrod dynamic injection is accomplished by the generation of membrane pores in the hepatocyte.

High levels of foreign gene expression in hepatocytes after tail vein injections of naked plasmid DNA.

It is demonstrated that high levels of plasmid DNA expression in hepatocytes can be easily obtained by tail vein injections and has great potential for a wide variety of laboratory studies.

Regional hydrodynamic gene delivery to the rat liver with physiological volumes of DNA solution

This work evaluated the regional hydrodynamic gene delivery via branches of the portal vein approach in a rat model for hepatitis B and found it to be effective and efficient in terms of volume and efficiency.

Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA

A hydrodynamics-based procedure for expressing transgenes in mice by systemic administration of plasmid DNA is developed and which can be used as an effective means for studying gene function, gene regulation and molecular pathophysiology through gene transfer, as well as for expressing proteins in animals.

Naked DNA delivered intraportally expresses efficiently in hepatocytes.

These unprecedented levels of foreign gene expression from naked plasmid DNA document the ability of parenchymal cells in vivo to take up naked DNA following intravascular delivery.

Hydrodynamics‐based procedure involves transient hyperpermeability in the hepatic cellular membrane: implication of a nonspecific process in efficient intracellular gene delivery

Possible mechanisms for the intracellular transport of naked pDNA by this procedure, a so‐called hydrodynamics‐based procedure, are investigated.