Mechanism of plasmid delivery by hydrodynamic tail vein injection. I. Hepatocyte uptake of various molecules

@article{Sebestyn2006MechanismOP,
  title={Mechanism of plasmid delivery by hydrodynamic tail vein injection. I. Hepatocyte uptake of various molecules},
  author={Magdolna G. Sebesty{\'e}n and Vladimir G. Budker and Tatiana Budker and Vladimir M. Subbotin and Guofeng Zhang and Sean D. Monahan and David L. Lewis and So C. Wong and James E. Hagstrom and J. A. Wolff},
  journal={The Journal of Gene Medicine},
  year={2006},
  volume={8}
}
The hydrodynamic tail vein (HTV) injection of naked plasmid DNA is a simple yet effective in vivo gene delivery method into hepatocytes. It is increasingly being used as a research tool to elucidate mechanisms of gene expression and the role of genes and their cognate proteins in the pathogenesis of disease in animal models. A greater understanding of its mechanism will aid these efforts and has relevance to macromolecular and nucleic acid delivery in general. 
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The goal was to study the uptake mechanism by following the morphological changes in the liver and correlate these with the fate of the injected plasmid DNA. Expand
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Hydrodynamic delivery of siRNA in a mouse model of sepsis.
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References

SHOWING 1-10 OF 47 REFERENCES
Mechanism of plasmid delivery by hydrodynamic tail vein injection. II. Morphological studies
TLDR
The goal was to study the uptake mechanism by following the morphological changes in the liver and correlate these with the fate of the injected plasmid DNA. Expand
Uptake by mouse liver and intracellular fate of plasmid DNA after a rapid tail vein injection of a small or a large volume
TLDR
An efficient gene transfer can be achieved in mouse liver by a rapid tail vein injection of a large volume of plasmid DNA solution (hydrodynamics‐based transfection) and it must be related to the uptake and intracellular fate of DNA. Expand
Hydrodynamic delivery of DNA
TLDR
The development of the procedures for hydrodynamic delivery will be described and the parameters necessary for attaining maximal gene expression will be highlighted, as well as potential uses of this approach in various research and clinical applications. Expand
Hydroporation as the mechanism of hydrodynamic delivery
TLDR
The hydrodynamic injection induces a transient irregularity of heart function, a sharp increase in venous pressure, an enlargement of liver fenestrae, and enhancement of membrane permeability of the hepatocytes, suggesting that hepatic delivery by the hydrod dynamic injection is accomplished by the generation of membrane pores in the hepatocyte. Expand
High levels of foreign gene expression in hepatocytes after tail vein injections of naked plasmid DNA.
TLDR
It is demonstrated that high levels of plasmid DNA expression in hepatocytes can be easily obtained by tail vein injections and has great potential for a wide variety of laboratory studies. Expand
Regional hydrodynamic gene delivery to the rat liver with physiological volumes of DNA solution
TLDR
This work evaluated the regional hydrodynamic gene delivery via branches of the portal vein approach in a rat model for hepatitis B and found it to be effective and efficient in terms of volume and efficiency. Expand
Hydrodynamics-based transfection of the liver: entrance into hepatocytes of DNA that causes expression takes place very early after injection.
TLDR
The results show that the efficiency of hydrodynamics-based transfection depends on a process that takes place very quickly after injection and is not linked to a delay of DNA degradation and the persistence of a large proportion of DNA bound to hepatocytes of the plasma membrane, strongly suggesting that expression after a hydrodynamic injection is caused by a small proportion ofDNA molecules that rapidly enter the cytosol probably by plasma membrane pores generated by the hydrod dynamic pressure. Expand
Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA
TLDR
A hydrodynamics-based procedure for expressing transgenes in mice by systemic administration of plasmid DNA is developed and which can be used as an effective means for studying gene function, gene regulation and molecular pathophysiology through gene transfer, as well as for expressing proteins in animals. Expand
Naked DNA delivered intraportally expresses efficiently in hepatocytes.
TLDR
These unprecedented levels of foreign gene expression from naked plasmid DNA document the ability of parenchymal cells in vivo to take up naked DNA following intravascular delivery. Expand
Hydrodynamics‐based procedure involves transient hyperpermeability in the hepatic cellular membrane: implication of a nonspecific process in efficient intracellular gene delivery
TLDR
Possible mechanisms for the intracellular transport of naked pDNA by this procedure, a so‐called hydrodynamics‐based procedure, are investigated. Expand
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