Mechanism of peroxide-inactivation of the sulphydryl enzyme glyceraldehyde-3-phosphate dehydrogenase.

  title={Mechanism of peroxide-inactivation of the sulphydryl enzyme glyceraldehyde-3-phosphate dehydrogenase.},
  author={C. Little and P. O'brien},
  journal={European journal of biochemistry},
  volume={10 3},
  • C. Little, P. O'brien
  • Published 1969
  • Chemistry, Medicine
  • European journal of biochemistry
  • The inactivation of glyceraldehyde-3-phosphate dehydrogenase by hydrogen peroxide has been investigated. The reaction obeyed two rate equations: (a) In the absence of catalysts (b) In the presence of catalysts These Kinetics are identical to those previously observed during glutathione oxidation by peroxides. Enzyme inactivation was shown to be strictly related to sulphydryl group modification and this modification can account for the inactivation… CONTINUE READING
    137 Citations
    Regulation of the activity of glyceraldehyde 3-phosphate dehydrogenase by glutathione and H2O2
    • 13
    Glyceraldehyde-3-phosphate dehydrogenase inactivation by peroxynitrite.
    • 155
    The inactivation of isocitrate dehydrogenase by a lipid peroxide.
    • 31
    The inactivation of papain and glyceraldehyde-3-phosphate dehydrogenase by phenyldiimide.
    • 7


    Products of oxidation of a protein thiol group after reaction with various oxidizing agents.
    • 48
    The mechanism of x-ray inactivation of phosphoglyceraldehyde dehydrogenase.
    • R. Lange, A. Pihl
    • Chemistry, Medicine
    • International journal of radiation biology and related studies in physics, chemistry, and medicine
    • 1960
    • 27
    Studies on the active--SH group of papain and on the mechanism of papain activation by thiols.
    • 49
    • PDF
    Tissue sulfhydryl groups.
    • G. Ellman
    • Chemistry, Medicine
    • Archives of biochemistry and biophysics
    • 1959
    • 19,450
    Protein measurement with the Folin phenol reagent.
    • 274,897