Mechanism of nucleotide binding to actomyosin VI: evidence for allosteric head-head communication.

@article{Robblee2004MechanismON,
  title={Mechanism of nucleotide binding to actomyosin VI: evidence for allosteric head-head communication.},
  author={James P Robblee and Adrian O. Olivares and Enrique M. De La Cruz},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 37},
  pages={38608-17}
}
We have examined the kinetics of nucleotide binding to actomyosin VI by monitoring the fluorescence of pyrene-labeled actin filaments. ATP binds single-headed myosin VI following a two-step reaction mechanism with formation of a low affinity collision complex (1/K(1)' = 5.6 mm) followed by isomerization (k(+2)' = 176 s-1) to a state with weak actin affinity. The rates and affinity for ADP binding were measured by kinetic competition with ATP. This approach allows a broader range of ADP… CONTINUE READING

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