Mechanism of metal-mediated DNA damage induced by metabolites of carcinogenic 2-nitropropane.

@article{Sakano2001MechanismOM,
  title={Mechanism of metal-mediated DNA damage induced by metabolites of carcinogenic 2-nitropropane.},
  author={Katsuhisa Sakano and S Oikawa and Mariko Murata and Yusuke Hiraku and Nakao Kojima and Shosuke Kawanishi},
  journal={Mutation research},
  year={2001},
  volume={479 1-2},
  pages={101-11}
}
2-Nitropropane (2-NP), a widely used industrial solvent, is carcinogenic to rats. To clarify the mechanism of carcinogenesis by 2-NP, we investigated DNA damage by 2-NP metabolites, N-isopropylhydroxylamine (IPHA) and hydroxylamine-O-sulfonic acid (HAS), using 32P-5'-end-labelled DNA fragments obtained from genes that are relevant to human cancer. In the presence of Fe(III) EDTA, both IPHA and HAS caused DNA damage at every nucleotide position without marked site preference. The damage was… CONTINUE READING

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