Mechanism of intracellular cAMP sensor Epac2 activation: cAMP-induced conformational changes identified by amide hydrogen/deuterium exchange mass spectrometry (DXMS).

@article{Li2011MechanismOI,
  title={Mechanism of intracellular cAMP sensor Epac2 activation: cAMP-induced conformational changes identified by amide hydrogen/deuterium exchange mass spectrometry (DXMS).},
  author={Sheng Li and Tamara N Tsalkova and Mark A White and Fang C. Mei and Tong Liu and Daphne W. Wang and Virgil L. Woods and Xiaodong Cheng},
  journal={The Journal of biological chemistry},
  year={2011},
  volume={286 20},
  pages={17889-97}
}
Epac2, a guanine nucleotide exchange factor, regulates a wide variety of intracellular processes in response to second messenger cAMP. In this study, we have used peptide amide hydrogen/deuterium exchange mass spectrometry to probe the solution structural and conformational dynamics of full-length Epac2 in the presence and absence of cAMP. The results support a mechanism in which cAMP-induced Epac2 activation is mediated by a major hinge motion centered on the C terminus of the second cAMP… CONTINUE READING
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