Mechanism of inhibition of skeletal muscle actomyosin by N-benzyl-p-toluenesulfonamide.

  title={Mechanism of inhibition of skeletal muscle actomyosin by N-benzyl-p-toluenesulfonamide.},
  author={M. Alexander Shaw and E Michael Ostap and Yale E. Goldman},
  volume={42 20},
N-Benzyl-p-toluenesulfonamide (BTS) is a small organic molecule that specifically inhibits the contraction of fast skeletal muscle fibers. To determine the mechanism of inhibition by BTS, we performed a kinetic analysis of its effects on the elementary steps of the actomyosin subfragment-1 ATPase cycle. BTS decreases the steady-state acto-S1 ATPase rate approximately 10-fold and increases the actin concentration for half-maximal activation. BTS primarily affects three of the elementary steps of… CONTINUE READING