Mechanism of hairpin-duplex conversion for the HIV-1 dimerization initiation site.

@article{Bernacchi2005MechanismOH,
  title={Mechanism of hairpin-duplex conversion for the HIV-1 dimerization initiation site.},
  author={Serena Bernacchi and Eric Ennifar and Katalin Toth and Philippe Walter and J{\"o}rg Langowski and Philippe Dumas},
  journal={The Journal of biological chemistry},
  year={2005},
  volume={280 48},
  pages={40112-21}
}
We have used the dimerization initiation site of HIV-1 genomic RNA as a model to investigate hairpin-duplex interconversion with a combination of fluorescence, UV melting, gel electrophoresis, and x-ray crystallographic techniques. Fluorescence studies with molecular beacons and crystallization experiments with 23-nucleotide dimerization initiation site fragments showed that the ratio of hairpin to duplex formed after annealing in water essentially depends on RNA concentration and not on… CONTINUE READING

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Nucleic Acids, Structure, Properties, and Functions (Bloomfield

D. H. Turner
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Highly Influenced

Hairpin-Duplex Conversion 40120 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME

S. Tishchenko, A. Nikulin, +3 authors P. Dumas
J. Mol. Biol • 2005

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