Mechanism of dye response and interference in the Bradford protein assay.

@article{Compton1985MechanismOD,
  title={Mechanism of dye response and interference in the Bradford protein assay.},
  author={Steve J. Compton and Clive G. Jones},
  journal={Analytical biochemistry},
  year={1985},
  volume={151 2},
  pages={369-74}
}
Bradford Coomassie brilliant blue G-250 protein-binding dye exists in three forms: cationic, neutral, and anionic. Although the anion is not freely present at the dye reagent pH, it is this form that complexes with protein. Dye binding requires a macromolecular form with certain reactive functional groups. Interactions are chiefly with arginine rather than primary amino groups; the other basic (His, Lys) and aromatic residues (Try, Tyr, and Phe) give slight responses. The binding behavior is… CONTINUE READING

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