Mechanism of direct coupling between binding and induced structural change in regulatory calcium binding proteins.

@article{Gagn1997MechanismOD,
  title={Mechanism of direct coupling between binding and induced structural change in regulatory calcium binding proteins.},
  author={St{\'e}phane M. Gagn{\'e} and Monica X. Li and Brian D. Sykes},
  journal={Biochemistry},
  year={1997},
  volume={36 15},
  pages={4386-92}
}
The structural transition in troponin C induced by the binding of two calcium ions involves an "opening" of the structure, an event that triggers skeletal muscle contraction. We have solved the solution structure of a mutant (E41A) of the regulatory domain of skeletal troponin C wherein one bidentate ligand to the calcium in site I is missing. This structure remains "closed" upon calcium binding, indicating that the linkage between calcium binding and the induced conformational change has been… CONTINUE READING