Mechanism of conjugation

  title={Mechanism of conjugation},
  author={Aleksandra Blinkowa and Grazyna Gawecka},
  journal={Molecular and General Genetics MGG},
A new conditional thermosensitive Hfr mutant of Escherichia coli K-12 was isolated. The ts mutation is cotransducible with purE and tsx loci on the E. coli chromosome. Upon temperature shift to 42° C the DNA synthesis and transfer of chromosome is stopped immediately and RNA, protein synthesis in about ten minutes.