Mechanism of conjugation

@article{Blinkowa2004MechanismOC,
  title={Mechanism of conjugation},
  author={Aleksandra Blinkowa and Grazyna Gawecka},
  journal={Molecular and General Genetics MGG},
  year={2004},
  volume={172},
  pages={107-111}
}
A new conditional thermosensitive Hfr mutant of Escherichia coli K-12 was isolated. The ts mutation is cotransducible with purE and tsx loci on the E. coli chromosome. Upon temperature shift to 42° C the DNA synthesis and transfer of chromosome is stopped immediately and RNA, protein synthesis in about ten minutes.