Mechanism of basic calcium phosphate crystal-stimulated matrix metalloproteinase-13 expression by osteoarthritic synovial fibroblasts: inhibition by prostaglandin E2

@article{Molloy2008MechanismOB,
  title={Mechanism of basic calcium phosphate crystal-stimulated matrix metalloproteinase-13 expression by osteoarthritic synovial fibroblasts: inhibition by prostaglandin E2},
  author={Eamonn S. Molloy and Maria P. Morgan and George A. Doherty and Barry J. McDonnell and John M. O'Byrne and Desmond J. Fitzgerald and Geraldine Mccarthy},
  journal={Annals of the Rheumatic Diseases},
  year={2008},
  volume={67},
  pages={1773 - 1779}
}
Objective: To determine the mechanism of matrix metalloproteinase (MMP)-13 upregulation in osteoarthritic synovial fibroblasts (OASF) in response to stimulation with basic calcium phosphate (BCP) crystals and to investigate the effect of prostaglandin (PG)E2 on BCP crystal-stimulated MMP expression. Methods: Primary OASF were stimulated with BCP crystals; mRNA expression was measured by real-time reverse transcription–polymerase chain reaction and protein levels were assessed by Western… 

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The ability of BCP to induce the synthesis of degradative MMPs while down-regulating the synthesisof the naturally occurring counterpart TIMPs may explain the changes consistent with a role of B CP crystal in the pathogenesis of degenerative changes in osteoarthritis.
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