Mechanism of alanine excretion in recombinant strains of Zymomonas mobilis.

@article{Ruhrmann1994MechanismOA,
  title={Mechanism of alanine excretion in recombinant strains of Zymomonas mobilis.},
  author={Josef Ruhrmann and Georg A. Sprenger and Reinhard Krämer},
  journal={Biochimica et biophysica acta},
  year={1994},
  volume={1196 1},
  pages={
          14-20
        }
}
A thiamine-auxotrophic strain of Zymomonas mobilis (CP4thi/pZY73), in which the alaD gene of Bacillus sphaericus coding for the alanine dehydrogenase was expressed, synthesizes and excretes alanine at high rates after thiamine starvation and in the presence of high external ammonium concentrations. The mechanism of alanine excretion was studied in this recombinant Zymomonas mobilis strain. Under production conditions the internal alanine concentration reached values of up to 280 mM and… Expand
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