Mechanism of action of cerulenin on fatty acid synthetase. Effect of cerulenin on iodoacetamide-induced malonyl-CoA decarboxylase activity.

@article{Kawaguchi1982MechanismOA,
  title={Mechanism of action of cerulenin on fatty acid synthetase. Effect of cerulenin on iodoacetamide-induced malonyl-CoA decarboxylase activity.},
  author={Akihiko Kawaguchi and Hiroshi Tomoda and Shigeo Nozoe and Satoshi O̅mura and Shigenobu Okuda},
  journal={Journal of biochemistry},
  year={1982},
  volume={92 1},
  pages={7-12}
}
Cerulenin, an antibiotic with the structure of (2R)(3S)-2,3-epoxy-4-oxo-7,10-dodecadienoylamide, irreversibly inactivates yeast fatty acid synthetase. Of all catalytic activities of the synthetase, only the condensation reaction is inhibited by cerulenin. At 0 degrees C and pH 6.5, the second-order rate constant of k = 88 M-1 . S-1 was obtained for the inactivation by cerulenin. This value was about 90-times greater than the rate constant for the inactivation of the enzyme by iodoacetamide. The… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 16 extracted citations

Similar Papers

Loading similar papers…