Mechanism of action of an acidic transcriptional activator in vitro

@article{Lin1991MechanismOA,
  title={Mechanism of action of an acidic transcriptional activator in vitro},
  author={Y Lin and Michael R. Green},
  journal={Cell},
  year={1991},
  volume={64},
  pages={971-981}
}
Transcription of a eukaryotic structural gene by RNA polymerase II requires the ordered assembly of general transcription factors on the promoter to form a pre-initiation complex. Here we analyze affinity-purified complexes at various stages of assembly to determine the mechanism of action of an acidic transcriptional activator. We show that the activator can function in the absence of ATP and stimulates transcription by increasing the number of functional preinitiation complexes. The activator… CONTINUE READING

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