Mechanism of action of a yeast RNA ligase in tRNA splicing

@article{Greer1983MechanismOA,
  title={Mechanism of action of a yeast RNA ligase in tRNA splicing},
  author={C. Greer and C. Peebles and P. Gegenheimer and J. Abelson},
  journal={Cell},
  year={1983},
  volume={32},
  pages={537-546}
}
  • C. Greer, C. Peebles, +1 author J. Abelson
  • Published 1983
  • Biology, Medicine
  • Cell
  • Abstract The yeast endonuclease and ligase activities that carry out the splicing of tRNA precursors in vitro have been physically separated. The properties of a partially purified ligase fraction were examined. The ligase requires a divalent cation and a nucleoside triphosphate as cofactor. The product of ligation is a 2′-phosphomonoester, 3′,5′-phosphodiester linkage. The phosphate in the newly formed phosphodiester bond comes from the γ position of ATP, while the 2′ phosphate is derived from… CONTINUE READING
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