Mechanism of action of a yeast RNA ligase in tRNA splicing

  title={Mechanism of action of a yeast RNA ligase in tRNA splicing},
  author={C. Greer and C. Peebles and P. Gegenheimer and J. Abelson},
  • C. Greer, C. Peebles, +1 author J. Abelson
  • Published 1983
  • Biology, Medicine
  • Cell
  • Abstract The yeast endonuclease and ligase activities that carry out the splicing of tRNA precursors in vitro have been physically separated. The properties of a partially purified ligase fraction were examined. The ligase requires a divalent cation and a nucleoside triphosphate as cofactor. The product of ligation is a 2′-phosphomonoester, 3′,5′-phosphodiester linkage. The phosphate in the newly formed phosphodiester bond comes from the γ position of ATP, while the 2′ phosphate is derived from… CONTINUE READING
    188 Citations
    Purification of yeast transfer RNA ligase.
    • 5
    Domain structure in yeast tRNA ligase.
    • 49
    The 2′-5′ RNA Ligase of Escherichia coli
    • 59
    • PDF
    Use of domain enzymes from wheat RNA ligase for in vitro preparation of RNA molecules.
    Genetic and Biochemical Analysis of the Functional Domains of Yeast tRNA Ligase*
    • 65
    • PDF
    Highly purified transfer RNA splicing endonuclease from Saccharomyces cerevisiae.
    • 2


    Splicing of yeast tRNA precursors: structure of the reaction intermediates
    • 89
    Origin of splice junction phosphate in tRNAs processed by HeLa cell extract
    • 120
    Bacteriophage T4 RNA ligase. Reaction intermediates and interaction of substrates.
    • 73
    Nuclear ligation of RNA 5'-OH kinase products in tRNA.
    • 17
    • PDF
    Yeast tRNA3Leu gene transcribed and spliced in a HeLa cell extract.
    • 26
    • PDF
    The enzymatic cleavage of phosphate termini from polynucleotides.
    • 46