Mechanism of action of a yeast RNA ligase in tRNA splicing

@article{Greer1983MechanismOA,
  title={Mechanism of action of a yeast RNA ligase in tRNA splicing},
  author={C. Greer and C. Peebles and P. Gegenheimer and J. Abelson},
  journal={Cell},
  year={1983},
  volume={32},
  pages={537-546}
}
  • C. Greer, C. Peebles, +1 author J. Abelson
  • Published 1983
  • Biology, Medicine
  • Cell
    • Abstract The yeast endonuclease and ligase activities that carry out the splicing of tRNA precursors in vitro have been physically separated. The properties of a partially purified ligase fraction were examined. The ligase requires a divalent cation and a nucleoside triphosphate as cofactor. The product of ligation is a 2′-phosphomonoester, 3′,5′-phosphodiester linkage. The phosphate in the newly formed phosphodiester bond comes from the γ position of ATP, while the 2′ phosphate is derived from… CONTINUE READING
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    188 Citations
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    The 2′-5′ RNA Ligase of Escherichia coli
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    Genetic and Biochemical Analysis of the Functional Domains of Yeast tRNA Ligase*
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    Precise excision of intervening sequences from precursor tRNAs by a membrane-associated yeast endonuclease
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    Highly purified transfer RNA splicing endonuclease from Saccharomyces cerevisiae.
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    An NAD derivative produced during transfer RNA splicing: ADP-ribose 1"-2" cyclic phosphate.
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    References

    SHOWING 1-10 OF 27 REFERENCES
    Precise excision of intervening sequences from precursor tRNAs by a membrane-associated yeast endonuclease
    • 173
    Splicing of yeast tRNA precursors: structure of the reaction intermediates
    • 89
    Origin of splice junction phosphate in tRNAs processed by HeLa cell extract
    • 120
    Studies on ribonucleic acid ligase. Characterization of an adenosine triphosphate-inorganic pyrophosphate exchange reaction and demonstration of an enzyme-adenylate complex with T4 bacteriophage-induced enzyme.
    • 72
    Splicing of yeast tRNA precursors: a two-stage reaction
    • 96
    Bacteriophage T4 RNA ligase. Reaction intermediates and interaction of substrates.
    • 73
    Nuclear ligation of RNA 5'-OH kinase products in tRNA.
    • 17
    • PDF
    Yeast tRNA3Leu gene transcribed and spliced in a HeLa cell extract.
    • 26
    • PDF
    Structure and processing of yeast precursor tRNAs containing intervening sequences
    • 90
    • PDF
    The enzymatic cleavage of phosphate termini from polynucleotides.
    • 46