Mechanism of action of a yeast RNA ligase in tRNA splicing
@article{Greer1983MechanismOA, title={Mechanism of action of a yeast RNA ligase in tRNA splicing}, author={C. Greer and C. Peebles and P. Gegenheimer and J. Abelson}, journal={Cell}, year={1983}, volume={32}, pages={537-546} }
Abstract The yeast endonuclease and ligase activities that carry out the splicing of tRNA precursors in vitro have been physically separated. The properties of a partially purified ligase fraction were examined. The ligase requires a divalent cation and a nucleoside triphosphate as cofactor. The product of ligation is a 2′-phosphomonoester, 3′,5′-phosphodiester linkage. The phosphate in the newly formed phosphodiester bond comes from the γ position of ATP, while the 2′ phosphate is derived from… CONTINUE READING
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