Mechanism of a Reaction in Vitro Associated with Delayed-Type Hypersensitivity

@article{Bloom1966MechanismOA,
  title={Mechanism of a Reaction in Vitro Associated with Delayed-Type Hypersensitivity},
  author={Barry R. Bloom and Boyce M. Bennett},
  journal={Science},
  year={1966},
  volume={153},
  pages={80 - 82}
}
The cell type responsible for inhibition by antigen of migration in vitro of peritoneal exudate cells obtained from tuberculin-hypersensitive guinea pigs was studied. Exudate populations were separated into component cell types, the lymphocyte and the macrophage. Peritoneal lymphocytes from sensitive donors were the immunologically active cells in this system, the macrophages, being merely indicator cells which migrate. Sensitized peritoneal lymphocyte populations, upon interaction with… 
Macrophage migration inhibition by serum from desensitized animals previously sensitized with tubercle bacilli.
TLDR
Peritoneal exudate cells removed from guinea-pigs or mice exhibiting delayed hypersensitivity are inhibited by specific antigen and lymphocytes from sensitized animals released into the medium a specific substance capable of inhibiting the migration of normal macrophages.
Cellular hypersensitivity: the production of antibody by cultures of lymphocytes producing macrophage inhibitory factor.
Abstract Lymph node lymphocytes from guinea pigs sensitized to bovine gamma globulin (BGG), when incubated with BGG elaborate a soluble material into the media which inhibits the migration of normal
In Vivo and In Vitro Studies of Delayed-Type Hypersensitivity to Toxoplasma gondii in Guinea Pigs
TLDR
Toxoplasma-infected guinea pigs were studied and both the toxoplasmin skin test and the in vitro macrophage migration inhibition technique indicated that delayed hypersensitivity to toxoplasma antigen existed as early as 1 week after infection.
Lymphoid cells in delayed hypersensitivity. 1. In vitro vs. in vivo responses.
TLDR
Passive transfer of delayed hypersensitivity to normal adult recipients was successful with cells from spleen, lymph nodes, and peritoneal exudate of adult sensitized guinea pigs but not with Cells from the thymus or bone marrow of the same donors.
Delayed Hypersensitivity in vitro
TLDR
Evidence has been found for a diffusible factor which is released from the sensitive cells in contact with the antigen; this factor inhibits migration of cells from a normal lymph node explant in vivo and in vitro.
Studies on mechanisms of immobilization of mononuclear cells in the delayed hypersensitivity (DH) reaction
Migration inhibitory (MI) activity in exudates, and ‘migration’ and ‘inhibition of migration’ of exudate cells was investigated in the delayed hypersensitivity (DH) reaction induced by intrapleural
Cytotoxic Effect of Lymphocyte-Antigen Interaction in Delayed Hypersensitivity
Lymph node cells from inbred rats having delayed sensitivity to soluble proteins inhibit growth of syngeneic or allogeneic fibroblasts in the presence of specific antigen. A relation is suggested
DEMONSTRATION OF DELAYED‐TYPE HYPERSENSITIVITY IN GUINEA‐PIGS BY THE AGAROSE PLATE TECHNIQUE
A recently developed method for demonstration of antigen-dependant migration inhibition, the agarose plate technique, and skin tests were used to demonstrate delayed hypersensitivity towards
Avian delayed-type hypersensitivity: adaptation of the migration-inhibition assay.
TLDR
The results of these experiments indicate that the delayed hypersensitive response in birds parallels that of the mammal in that it is antigen specific, reproducible, independent of the antibody response, and transferable to normal cells with a soluble cell-free product.
In citro migration of peripheral human leucocytes in cellular hypersensitivity.
  • M. Søborg
  • Biology, Medicine
    Acta medica Scandinavica
  • 1968
The influence of various antigen concentrations upon the in vitro migration of peripheral leucocytes from persons with cellular hypersensitivity to B. abortus Bang is investigated. An inhibition as
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References

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Summary 1.The migration of peritoneal exudate cells from guinea pigs with delayed hypersensitivity to tuberculin purified protein derivative, ovalbumin and diphtheria toxoid is markedly inhibited by
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TLDR
The ability of antigen to inhibit cell migration in tissue culture has been confirmed with peritoneal exudate cells for tuberculin hypersensitivity and this in vitro characteristic has been shown to apply also to the delayed hypersensitivity that develops after immunization with a protein antigen in Freund's adjuvant.
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TLDR
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TLDR
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Effects of Mitomycin C on the Cellular Transfer of Delayed-Type Hypersensitivity in the Guinea Pig
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