Mechanism of Phosphoanhydride Cleavage by Baculovirus Phosphatase*

@article{Martins2000MechanismOP,
  title={Mechanism of Phosphoanhydride Cleavage by Baculovirus Phosphatase*},
  author={A. Martins and S. Shuman},
  journal={The Journal of Biological Chemistry},
  year={2000},
  volume={275},
  pages={35070 - 35076}
}
  • A. Martins, S. Shuman
  • Published 2000
  • Biology, Medicine
  • The Journal of Biological Chemistry
  • Baculovirus phosphatase (BVP) is a member of the metazoan RNA triphosphatase enzyme family that includes the RNA triphosphatase component of the mRNA capping apparatus. BVP and other metazoan RNA triphosphatases belong to a superfamily of phosphatases that act via the formation and hydrolysis of a covalent cysteinyl-phosphate intermediate. Here we demonstrate the formation of a BVP phosphoenzyme upon reaction with [γ-32P]ATP and identify the linkage as a thiophosphate based on its chemical… CONTINUE READING
    24 Citations
    Crystal Structure of Baculovirus RNA Triphosphatase Complexed with Phosphate*
    • 25
    • PDF
    Characterization of Schizosaccharomyces pombe RNA triphosphatase.
    • 42
    • PDF
    A Plasmodium falciparum protein related to fungal RNA 5'-triphosphatases.
    • 7
    • PDF
    Magnesium-binding studies reveal fundamental differences between closely related RNA triphosphatases
    • 3
    • PDF
    The Caenorhabditis elegans mRNA 5'-capping enzyme. In vitro and in vivo characterization.
    • 20
    • PDF
    Structure-Function Analysis of the Active Site Tunnel of Yeast RNA Triphosphatase*
    • 43
    • PDF
    Structural and biochemical characterization of Siw14: A protein-tyrosine phosphatase fold that metabolizes inositol pyrophosphates
    • 6
    • PDF

    References

    SHOWING 1-10 OF 39 REFERENCES
    Characterization of a Baculovirus-Encoded RNA 5′-Triphosphatase
    • 42
    • PDF
    The Cys(X)5Arg catalytic motif in phosphoester hydrolysis.
    • 137
    Phylogeny of mRNA capping enzymes.
    • 105
    • PDF
    Mammalian capping enzyme binds RNA and uses protein tyrosine phosphatase mechanism.
    • 55
    • PDF
    A protein tyrosine phosphatase-like protein from baculovirus has RNA 5'-triphosphatase and diphosphatase activities.
    • 46
    • PDF
    Dissecting the catalytic mechanism of protein-tyrosine phosphatases.
    • Z. Zhang, Y. Wang, J. Dixon
    • Biology, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 1994
    • 219
    • PDF
    The catalytic role of Cys124 in the dual specificity phosphatase VHR.
    • 106