Mechanism of Phosphoanhydride Cleavage by Baculovirus Phosphatase*

  title={Mechanism of Phosphoanhydride Cleavage by Baculovirus Phosphatase*},
  author={A. Martins and S. Shuman},
  journal={The Journal of Biological Chemistry},
  pages={35070 - 35076}
  • A. Martins, S. Shuman
  • Published 2000
  • Biology, Medicine
  • The Journal of Biological Chemistry
  • Baculovirus phosphatase (BVP) is a member of the metazoan RNA triphosphatase enzyme family that includes the RNA triphosphatase component of the mRNA capping apparatus. BVP and other metazoan RNA triphosphatases belong to a superfamily of phosphatases that act via the formation and hydrolysis of a covalent cysteinyl-phosphate intermediate. Here we demonstrate the formation of a BVP phosphoenzyme upon reaction with [γ-32P]ATP and identify the linkage as a thiophosphate based on its chemical… CONTINUE READING
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