Mechanism of Gene Amplification via Yeast Autonomously Replicating Sequences

  title={Mechanism of Gene Amplification via Yeast Autonomously Replicating Sequences},
  author={Shelly Sehgal and Sanjana Kaul and Manoj Kumar Dhar},
The present investigation was aimed at understanding the molecular mechanism of gene amplification. Interplay of fragile sites in promoting gene amplification was also elucidated. The amplification promoting sequences were chosen from the Saccharomyces cerevisiae ARS, 5S rRNA regions of Plantago ovata and P. lagopus, proposed sites of replication pausing at Ste20 gene locus of S. cerevisiae, and the bend DNA sequences within fragile site FRA11A in humans. The gene amplification assays showed… CONTINUE READING
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