Mechanism of Divergent Growth Factor Effects in Mesenchymal Stem Cell Differentiation

@article{Kratchmarova2005MechanismOD,
  title={Mechanism of Divergent Growth Factor Effects in Mesenchymal Stem Cell Differentiation},
  author={Irina Kratchmarova and Blagoy S. Blagoev and Mandana Haack-S{\o}rensen and Moustapha Kassem and Matthias Mann},
  journal={Science},
  year={2005},
  volume={308},
  pages={1472 - 1477}
}
Closely related signals often lead to very different cellular outcomes. We found that the differentiation of human mesenchymal stem cells into bone-forming cells is stimulated by epidermal growth factor (EGF) but not platelet-derived growth factor (PDGF). We used mass spectrometry–based proteomics to comprehensively compare proteins that were tyrosine phosphorylated in response to EGF and PDGF and their associated partners. More than 90% of these signaling proteins were used by both ligands… Expand
Platelet-derived growth factor receptor signaling is not involved in osteogenic differentiation of human mesenchymal stem cells.
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Effects on Proliferation and Differentiation of Multipotent Bone Marrow Stromal Cells Engineered to Express Growth Factors for Combined Cell and Gene Therapy
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Effects of overexpressing the growth factors, such as basic fibroblast growth factor (bFGF), platelet derived growth factor B (PDGF‐BB), transforming growth factor β1 (TGF‐β1), and vascular endothelial growth factors (VEGF), in human bone marrow‐derived MSCs are shown. Expand
Serum Free Cultured Bone Marrow Mesenchymal Stem Cells as a Platform to Characterize the Effects of Specific Molecules
Human mesenchymal stem cells (hMSC) are easily isolated from the bone marrow by adherence to plastic surfaces. These cells show self-renewal capacity and multipotency. A unique feature of hMSC isExpand
Cross-talk between EGF and BMP9 signalling pathways regulates the osteogenic differentiation of mesenchymal stem cells
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  • Biology, Medicine
  • Journal of cellular and molecular medicine
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It is found that EGF potentiates BMP9‐induced early and late osteogenic markers of MSCs in vitro, which can be effectively blunted by EGFR inhibitors Gefitinib and Erlotinib or receptor tyrosine kinase inhibitors AG‐1478 and AG‐494 in a dose‐ and time‐dependent manner. Expand
Differential expression of cell surface proteins in human bone marrow mesenchymal stem cells cultured with or without basic fibroblast growth factor containing medium
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The results indicate that the expression levels of F‐actin‐capping protein subunit alpha‐1, actin‐related protein 2/3 complex subunit 2, and myosin regulatory light chain 2 are important in bFGF‐induced morphological change of MSCs. Expand
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Multipathway Kinase Signatures of Multipotent Stromal Cells Are Predictive for Osteogenic Differentiation
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A multivariate systems approach is undertaken integrating experimental measurement of multiple kinase pathway activities and osteogenic differentiation in MSCs, together with computational analysis to elucidate quantitative combinations of kinase signals predictive of cell behavior across diverse contexts. Expand
Quantitative analysis of the responses of murine bone marrow mesenchymal stem cells to EGF, PDGF-BB and fibronectin by factorial design methodology
A 2-level full factorial design was firstly employed to explore the responses of murine bone marrow mesenchymal stem cells stimulated by various combinations of EGF, PDGF-BB, and fibronectin. EGF andExpand
Involvement of PI3K and MMP1 in PDGF-induced Migration of Human Adipose-derived Stem Cells
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The results suggest that PDGF might signal hADSCs through PI3K, and MMP1 activity could play an important role in this PDGF-induced migration in vitro. Expand
PDGF Receptor β Is a Potent Regulator of Mesenchymal Stromal Cell Function
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  • Biology, Medicine
  • Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
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TLDR
PDGFRβ signaling distinctively induces proliferative and migratory responses but strongly inhibits osteogenic differentiation of MSCs, which could represent an important target for guided tissue regeneration or tissue engineering of bone. Expand
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