Mechanism of CO oxidation by carbon monoxide dehydrogenase from Clostridium thermoaceticum and its inhibition by anions.

@article{Seravalli1995MechanismOC,
  title={Mechanism of CO oxidation by carbon monoxide dehydrogenase from Clostridium thermoaceticum and its inhibition by anions.},
  author={Javier G. Seravalli and Manoj Kumar and W. P. Lu and Stephen W Ragsdale},
  journal={Biochemistry},
  year={1995},
  volume={34 24},
  pages={
          7879-88
        }
}
Carbon monoxide dehydrogenase (CODH) performs two distinct reactions at two different metal centers. The synthesis of acetyl-CoA from a methyl group, CO, and coenzyme A occurs at center A and the oxidation of CO to CO2 occurs at center C. In the work reported here, we have studied the mechanism of CO oxidation by CODH and its inhibition by thiocyanate. Our data are consistent with a ping-pong mechanism. A scheme to explain the first half-reaction was developed that includes binding of water and… CONTINUE READING