Mechanism of Association and Reciprocal Activation of Two GTPases

  title={Mechanism of Association and Reciprocal Activation of Two GTPases},
  author={Shu-Ou Shan and Robert Michael Stroud and Peter J. Walter},
  journal={PLoS Biology},
  pages={117 - 127}
The signal recognition particle (SRP) mediates the cotranslational targeting of nascent proteins to the eukaryotic endoplasmic reticulum membrane or the bacterial plasma membrane. During this process, two GTPases, one in SRP and one in the SRP receptor (named Ffh and FtsY in bacteria, respectively), form a complex in which both proteins reciprocally activate the GTPase reaction of one another. Here, we explore by site-directed mutagenesis the role of 45 conserved surface residues in the Ffh… CONTINUE READING