Mechanism of Agrobacterium beta-glucosidase: kinetic studies.

  title={Mechanism of Agrobacterium beta-glucosidase: kinetic studies.},
  author={Julia Beth Kempton and Stephen G Withers},
  volume={31 41},
The beta-glucosidase from Agrobacterium faecalis (previously Alcaligenes faecalis) has been subjected to a detailed kinetic investigation with a range of substrates to probe its specificity and mechanism. It has a relatively broad specificity for the substrate sugar moiety and exhibits a classical pH dependence for its kinetic parameters with three different substrates and an identical pH dependence for its inactivation by a mechanism-based inactivator, cyclophellitol. Measurement of kcat and… CONTINUE READING


Publications citing this paper.
Showing 1-10 of 39 extracted citations

Similar Papers

Loading similar papers…