Mechanism of Action of Eosin-Methylene Blue Agar in the Differentiation of Escherichia coli and Enterobacter aerogenes

  title={Mechanism of Action of Eosin-Methylene Blue Agar in the Differentiation of Escherichia coli and Enterobacter aerogenes},
  author={R. Horvath and M. Ropp},
  journal={International Journal of Systematic and Evolutionary Microbiology},
  • R. Horvath, M. Ropp
  • Published 1 April 1974
  • Biology
  • International Journal of Systematic and Evolutionary Microbiology
The mechanism involved in the action of eosin-methylene blue agar (EMB) in differentiating Escherichia coli from Enterobacter aerogenes was investigated using prototrophic strains of these organisms and a Lac − auxotroph, E. coli X-961. [...] Key Result An EMB complex, which formed under acidic conditions, appeared to be involved in the differential action of this medium. The molecular weight of the complex indicated an eosin to methylene blue ratio of 1 to 1.Expand

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