Mechanism for activation of mouse mast cell tryptase: dependence on heparin and acidic pH for formation of active tetramers of mouse mast cell protease 6.

@article{Hallgren2000MechanismFA,
  title={Mechanism for activation of mouse mast cell tryptase: dependence on heparin and acidic pH for formation of active tetramers of mouse mast cell protease 6.},
  author={Jenny Hallgren and Ulrika Karlson and Maryam Poorafshar and Lars Hellman and Gunnar Pejler},
  journal={Biochemistry},
  year={2000},
  volume={39 42},
  pages={13068-77}
}
Tryptase, a serine protease with trypsin-like substrate cleavage properties, is one of the key effector molecules during allergic inflammation. It is stored in large quantities in the mast cell secretory granules in complex with heparin proteoglycan, and these complexes are released during mast cell degranulation. In the present paper, we have studied the mechanism for tryptase activation. Recombinant mouse tryptase, mouse mast cell protease 6 (mMCP-6), was produced in a mammalian expression… CONTINUE READING