Mechanism and utility of an RNA-cleaving DNA enzyme.
@article{Santoro1998MechanismAU,
title={Mechanism and utility of an RNA-cleaving DNA enzyme.},
author={S. Santoro and G. F. Joyce},
journal={Biochemistry},
year={1998},
volume={37 38},
pages={
13330-42
}
}We previously reported the in vitro selection of a general-purpose RNA-cleaving DNA enzyme that exhibits a catalytic efficiency (kcat/KM) exceeding that of any other known nucleic acid enzyme [Santoro, S. W. and Joyce, G. F. (1997) Proc. Natl. Acad. Sci. U.S.A. 94, 4262-4266]. This enzyme contains approximately 30 deoxynucleotides and can cleave almost any RNA substrate under simulated physiological conditions, recognizing the substrate through two Watson-Crick binding domains. The kinetics of… CONTINUE READING
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